概述

  • 产品名称Anti-eIF2B epsilon抗体
    参阅全部 eIF2B epsilon 一抗
  • 描述
    兔多克隆抗体to eIF2B epsilon
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human eIF2B epsilon.

    (Peptide available as ab32712.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) Brain (Mouse and Rat)Tissue lysates Liver (Mouse and Rat)Tissue lysates

性能

应用

Our Abpromise guarantee covers the use of ab32713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
  • 疾病相关Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
  • 序列相似性Belongs to the eIF-2B gamma/epsilon subunits family.
    Contains 1 W2 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CACH antibody
    • CLE antibody
    • EI2BE_HUMAN antibody
    • EIF 2B antibody
    • eIF 2B GDP GTP exchange factor antibody
    • eIF 2B GDP GTP exchange factor subunit epsilon antibody
    • eIF-2B GDP-GTP exchange factor subunit epsilon antibody
    • EIF2B antibody
    • EIF2B5 antibody
    • EIF2BE antibody
    • EIF2Bepsilon antibody
    • Eukaryotic Translation Initiation Factor 2 Beta Epsilon antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon antibody
    • LVWM antibody
    • Translation initiation factor eIF 2B subunit epsilon antibody
    • Translation initiation factor eIF-2B subunit epsilon antibody
    see all

Anti-eIF2B epsilon antibody 图像

  • All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible cleavage fragment),73 kDa (possible cleavage fragment).
  • All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/250 dilution

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 7 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible cleavage fragment).
  • ICC/IF image of ab32713 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32713, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ICC/IF image of ab32713 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32713, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-eIF2B epsilon antibody (ab32713)参考文献

This product has been referenced in:
  • Toth MJ  et al. Chronic heart failure reduces Akt phosphorylation in human skeletal muscle: relationship to muscle size and function. J Appl Physiol 110:892-900 (2011). Read more (PubMed: 21193562) »
  • Glover EI  et al. Resistance exercise decreases eIF2B{epsilon} phosphorylation and potentiates the feeding-induced stimulation of p70S6k1 and rpS6 in young men. Am J Physiol Regul Integr Comp Physiol : (2008). Read more (PubMed: 18565837) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Placenta)
Loading amount 50 µg
Specification Placenta
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 09 2008

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