Anti-EIF2AK1抗体(ab84980)

概述

  • 产品名称Anti-EIF2AK1抗体
    参阅全部 EIF2AK1 一抗
  • 描述
    兔多克隆抗体to EIF2AK1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 107-156 (TCSDEFSSLR LHHNRAITHL MRSAKERVRQ DPCEDISRIQ KIRSREVALE) of Human EIF2AK1 (NP_055228).

  • 阳性对照
    • Fetal lung lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
  • 组织特异性Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 HRM (heme regulatory motif) repeats.
    Contains 1 protein kinase domain.
  • 翻译后修饰Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E2AK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak1 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 1 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 1 antibody
    • HCR antibody
    • Heme controlled repressor antibody
    • Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody
    • Heme regulated inhibitor antibody
    • Heme regulated initiation factor 2 alpha kinase antibody
    • Heme regulated repressor antibody
    • Heme sensitive initiation factor 2a kinase antibody
    • Heme-controlled repressor antibody
    • Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody
    • Heme-regulated inhibitor antibody
    • Hemin sensitive initiation factor 2 alpha kinase antibody
    • Hemin-sensitive initiation factor 2-alpha kinase antibody
    • HRI antibody
    • KIAA1369 antibody
    • PRO1362 antibody
    see all

Anti-EIF2AK1 antibody 图像

  • ab84980 staining EIF2AK1 in lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84980 staining EIF2AK1 in brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-EIF2AK1 antibody (ab84980)参考文献

ab84980 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (7.5)
Sample Human Cell lysate - whole cell (HEK293T cell line)
Specification HEK293T cell line
Blocking step Blocking One as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"