Anti-eIF2A抗体(ab55924)

概述

  • 产品名称Anti-eIF2A抗体
    参阅全部 eIF2A 一抗
  • 描述
    兔多克隆抗体to eIF2A
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: DKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQ , corresponding to C terminal amino acids 505-554 of Human eIF2A.

  • 阳性对照
    • Fetal thymus cell lysate and skin.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab55924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

靶标

Anti-eIF2A antibody 图像

  • Anti-eIF2A antibody (ab55924) at 2.5 µg/ml + Fetal thymus cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling eIF2A with ab55924 at 4-8µg/ml. Arrows indicate positively labelled epidermal cells. Magnification: 400X.

Anti-eIF2A antibody (ab55924)参考文献

ab55924 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam and thank you for bringing these issue to us. It does appear that these products should be listed as against eIF2A instead of eIF2 subunit alpha. I will make sure that this is brought to the attention of the appropria...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"