The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Antigen retrieval is recommended. Antigen retrieval with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.
功能Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
组织特异性Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
序列相似性Belongs to the WD repeat EIF2A family. Contains 3 WD repeats.