概述

  • 产品名称Anti-EID1抗体[26]
    参阅全部 EID1 一抗
  • 描述
    小鼠单克隆抗体[26] to EID1
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    YEKTPFDQLAFIEELFSLMVVNRLTEELGC

    , corresponding to amino acids 159 - 187 of Human EID1

  • 阳性对照
    • extracts from MCF7 cells transfected with EID1
  • 常规说明Clone number: #26

性能

应用

Our Abpromise guarantee covers the use of ab78323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.

靶标

  • 功能Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
  • 组织特异性Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.
  • 发展阶段Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).
  • 翻译后修饰Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
  • 细胞定位Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 21 kDa pRb associated protein antibody
    • 21 kDa pRb-associated protein antibody
    • C15orf3 antibody
    • CBP antibody
    • Chromosome 15 open reading frame 3 antibody
    • CREBBP/EP300 inhibitor 1 antibody
    • CREBBP/EP300 inhibitory protein 1 antibody
    • CRI 1 antibody
    • CRI1 antibody
    • E1A like inhibitor of differentiation antibody
    • E1A-like inhibitor of differentiation 1 antibody
    • EID 1 antibody
    • EID-1 antibody
    • EID1 antibody
    • EID1_HUMAN antibody
    • EP300 interacting inhibitor of differentiation 1 antibody
    • EP300-interacting inhibitor of differentiation 1 antibody
    • IRO45620 antibody
    • MGC138883 antibody
    • MGC138884 antibody
    • NB4 apoptosis related protein antibody
    • PNAS 22 antibody
    • PNAS22 antibody
    • PTD014 antibody
    • Rb and p300 binding protein EID 1 antibody
    • Rb and p300 binding protein EID1 antibody
    • RBP21 antibody
    • Retinoblastoma protein associated protein antibody
    see all

Anti-EID1 antibody [26] 图像

  • ab78323 staining EID1 in HeLA cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with Triton X-100 0.25% in PBS and blocked with 1.5% BSA for 30 minutes. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) overnight at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/1000 in PBS + 1% BSA) was used as the secondary antibody incubated for 1 hour. DAPI was used to stain the nuclear DNA.

  • All lanes : Anti-EID1 antibody [26] (ab78323) at 1 µg/ml

    Lane 1 : extracts from MCF7 cells transfected with control vector pCMV1
    Lane 2 : extracts from MCF7 cells transfected with the EID1 expression vector pcDNA3/EID1

    Secondary
    HRP-conjugated mouse IgG

    Predicted band size : 21 kDa
    Observed band size : 21 kDa

Anti-EID1 antibody [26] (ab78323)参考文献

ab78323 has not yet been referenced specifically in any publications.

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