Anti-EHMT1/GLP抗体[B0422] - ChIP Grade (ab41969)

概述

  • 产品名称
    Anti-EHMT1/GLP抗体[B0422] - ChIP Grade
    参阅全部 EHMT1/GLP 一抗
  • 描述
    小鼠单克隆抗体[B0422] to EHMT1/GLP - ChIP Grade
  • 经测试应用
    适用于: ChIP, ICC/IF, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Mouse EHMT1/GLP aa 134-234.
    Database link: Q5DW34

  • 阳性对照
    • WT mouse ES cell

性能

应用

Our Abpromise guarantee covers the use of ab41969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 18066052
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa.
ELISA Use a concentration of 0.1 µg/ml.
IP Use at an assay dependent dilution.

靶标

  • 功能
    Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
  • 组织特异性
    Widely expressed.
  • 疾病相关
    Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
  • 序列相似性
    Belongs to the histone-lysine methyltransferase family.
    Contains 8 ANK repeats.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • 结构域
    The ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
    The SET domain mediates interaction with WIZ.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Chromosome. Associates with euchromatic regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA188C12.1 antibody
    • DKFZp667M072 antibody
    • EHMT 1 antibody
    • EHMT1 antibody
    • EHMT1_HUMAN antibody
    • Eu-HMTase1 antibody
    • Euchromatic histone lysine N methyltransferase 1 antibody
    • Euchromatic histone methyltransferase 1 antibody
    • Euchromatic histone-lysine N-methyltransferase 1 antibody
    • EUHMTASE1 antibody
    • FLJ12879 antibody
    • FP13812 antibody
    • G9a like protein 1 antibody
    • G9a like protein antibody
    • G9a-like protein 1 antibody
    • GLP 1 antibody
    • GLP antibody
    • GLP1 antibody
    • H3 K9 HMTase 5 antibody
    • H3-K9-HMTase 5 antibody
    • Histone H3 K9 methyltransferase 5 antibody
    • Histone H3-K9 methyltransferase 5 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 5 antibody
    • Histone-lysine N-methyltransferase EHMT1 antibody
    • KIAA1876 antibody
    • Lysine N methyltransferase 1D antibody
    • Lysine N-methyltransferase 1D antibody
    • RP11 188C12.1 antibody
    see all

Anti-EHMT1/GLP antibody [B0422] - ChIP Grade 图像

  • Ab41969 at 5 µg/ml staining WT mouse ES cell (Positive)
  • Ab41969 at 5µg/ml staining GLP KO mouse ES cell (Negative)

Anti-EHMT1/GLP antibody [B0422] - ChIP Grade (ab41969)参考文献

This product has been referenced in:
  • Ow JR  et al. G9a inhibits MEF2C activity to control sarcomere assembly. Sci Rep 6:34163 (2016). IP . Read more (PubMed: 27667720) »
  • Balemans MC  et al. Reduced Euchromatin histone methyltransferase 1 causes developmental delay, hypotonia, and cranial abnormalities associated with increased bone gene expression in Kleefstra syndrome mice. Dev Biol 386:395-407 (2014). Mouse . Read more (PubMed: 24362066) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Dermal Microvascular Endothelial Cells)
Permeabilization
Yes - 0.1% Triton-X100
Specification
Human Dermal Microvascular Endothelial Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (B cells)
Negative control
IgG
Specification
B cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
Gene Promoter
Username

Abcam user community

Verified customer

提交于 Apr 27 2017

Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (Dermal Endothelial Cells (Primary))
Total protein in input
300 µg
Immuno-precipitation step
Protein A/G
Specification
Dermal Endothelial Cells (Primary)
Username

Abcam user community

Verified customer

提交于 Apr 24 2017

Application
Western blot
Sample
Human Cell lysate - nuclear (Dermal Endothelial cells (Primary))
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Dermal Endothelial cells (Primary)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 21 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C2C12 myoblast cells)
Specification
C2C12 myoblast cells
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 07 2013

Thank you so much for your reply.
Below are the testing discount codes for both GLP antibodies. At the bottom of the email is the information about the free of charge replacement for ab41969. A PO number is not needed on my end for sending you the...

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Thank you for your reply.
Yes, I think you are understanding this correctly.
I will be able to replace ab41969 free of charge with one vial of either ab63161 or ab84751. I am not able to send you both. Unfortunately, we do not offer sample si...

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Thank you for your reply and the additional information which will be very helpful to us to evaluate the antibody.
I have checked, but unfortunately we do not have another new lot for ab41969 in stock right now. Instead, I can send you a replaceme...

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Thank you for contacting us.
I am very sorry to hear that the antibody ab41969 is giving you problems.
Could you please let me know the following details for this antibody:
1) How were your samples prepared (e.g. lysis buffer)?
2) H...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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