Anti-EHMT1/GLP抗体(ab84751)

概述

  • 产品名称Anti-EHMT1/GLP抗体
    参阅全部 EHMT1/GLP 一抗
  • 描述
    兔多克隆抗体to EHMT1/GLP
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human EHMT1/GLP aa 175-225.
    Database link: Q9H9B1

  • 阳性对照
    • Human Breast Carcinoma, Ovarian Carcinoma and Prostate Carcinoma tissues.
  • 常规说明Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Centrifuge microtube to remove product from lid.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab84751 was affinity purified using an epitope specific to EHMT1/GLP immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.

Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections.

靶标

  • 功能Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
  • 组织特异性Widely expressed.
  • 疾病相关Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.
  • 序列相似性Belongs to the histone-lysine methyltransferase family.
    Contains 8 ANK repeats.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • 结构域The ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
    The SET domain mediates interaction with WIZ.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Chromosome. Associates with euchromatic regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA188C12.1 antibody
    • DKFZp667M072 antibody
    • EHMT 1 antibody
    • EHMT1 antibody
    • EHMT1_HUMAN antibody
    • Eu-HMTase1 antibody
    • Euchromatic histone lysine N methyltransferase 1 antibody
    • Euchromatic histone methyltransferase 1 antibody
    • Euchromatic histone-lysine N-methyltransferase 1 antibody
    • EUHMTASE1 antibody
    • FLJ12879 antibody
    • FP13812 antibody
    • G9a like protein 1 antibody
    • G9a like protein antibody
    • G9a-like protein 1 antibody
    • GLP 1 antibody
    • GLP antibody
    • GLP1 antibody
    • H3 K9 HMTase 5 antibody
    • H3-K9-HMTase 5 antibody
    • Histone H3 K9 methyltransferase 5 antibody
    • Histone H3-K9 methyltransferase 5 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 5 antibody
    • Histone-lysine N-methyltransferase EHMT1 antibody
    • KIAA1876 antibody
    • Lysine N methyltransferase 1D antibody
    • Lysine N-methyltransferase 1D antibody
    • RP11 188C12.1 antibody
    see all

Anti-EHMT1/GLP antibody 图像

  • ab84751 at 1/100 dilution staining EHMT1/GLP in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

  • ab84751 at 1/500 dilution staining EHMT1/GLP in human ovarian carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-EHMT1/GLP antibody (ab84751)参考文献

ab84751 has not yet been referenced specifically in any publications.

Product Wall

Thank you so much for your reply.
Below are the testing discount codes for both GLP antibodies. At the bottom of the email is the information about the free of charge replacement for ab41969. A PO number is not needed on my end for sending you the...

Read More

Thank you for your reply.
Yes, I think you are understanding this correctly.
I will be able to replace ab41969 free of charge with one vial of either ab63161 or ab84751. I am not able to send you both. Unfortunately, we do not offer sample si...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"