概述

  • 产品名称Anti-EHHADH抗体
    参阅全部 EHHADH 一抗
  • 描述
    兔多克隆抗体to EHHADH
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 673-723 of Human EHHADH using the numbering given in entry NP_001957.2.

  • 阳性对照
    • Whole cell lysate from 293T, Jurkat, HeLa

性能

应用

Our Abpromise guarantee covers the use of ab123490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2500. Predicted molecular weight: 79 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 组织特异性Liver and kidney. Lower amounts seen in the brain.
  • 通路Lipid metabolism; fatty acid beta-oxidation.
  • 序列相似性In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • 翻译后修饰Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
  • 细胞定位Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 hydroxyacyl CoA dehydrogenase antibody
    • 3,2 trans enoyl CoA isomerase antibody
    • 3-hydroxyacyl-CoA dehydrogenase antibody
    • ECHD antibody
    • ECHP_HUMAN antibody
    • EHHADH antibody
    • Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • L 3 hydroxyacyl CoA dehydrogenase antibody
    • L bifunctional protein, peroxisomal antibody
    • L PBE antibody
    • LBFP antibody
    • LBP antibody
    • MGC120586 antibody
    • MS730 antibody
    • PBE antibody
    • PBFE antibody
    • Peroxisomal bifunctional enzyme antibody
    • Peroxisomal enoyl CoA hydratase antibody
    see all

Anti-EHHADH antibody 图像

  • All lanes : Anti-EHHADH antibody (ab123490) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 79 kDa


    Exposure time : 3 minutes
  • Lane 1: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of EHHADH.
    Lane 2: Detection of Human EHHADH by Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123490 at 6 µg/mg lysate for IP.
    Lane 3: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab123490 at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-EHHADH antibody (ab123490)参考文献

ab123490 has not yet been referenced specifically in any publications.

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