Anti-EHF抗体(ab24337)

概述

  • 产品名称Anti-EHF抗体
    参阅全部 EHF 一抗
  • 描述
    兔多克隆抗体to EHF
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: PFQEFDINGE HLCSMSLQEF TRAAGTAGQL LYSNLQHLKW NGQCSSDLFQ, corresponding to N terminal amino acids 74-123 of Human EHF

性能

应用

Our Abpromise guarantee covers the use of ab24337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
  • 组织特异性Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 结构域The PNT domain acts as a transcriptional activator.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EHF antibody
    • EHF_HUMAN antibody
    • Epithelium-specific Ets transcription factor 3 antibody
    • ESE-3 antibody
    • ESE3 antibody
    • ESE3 transcription factor antibody
    • ESEJ antibody
    • Ets domain transcription factor antibody
    • ETS domain-containing transcription factor antibody
    • ETS homologous factor antibody
    • hEHF antibody
    see all

Anti-EHF antibody 图像

  • Anti-EHF antibody (ab24337) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000-1/100000

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    ab24337 was used at a dilution of 1.25 ug/ml in 5% skim milk / PBS buffer, with a secondary HRP conjugated anti-Rabbit IgG. Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EHF with ab24337 at 8µg/ml. Renal tubule epithelial cells labelled are indicated with arrows. Magnification: X400.

Anti-EHF antibody (ab24337)参考文献

ab24337 has not yet been referenced specifically in any publications.

Product Wall

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this ca...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"