概述

  • 产品名称Anti-EHD3抗体
    参阅全部 EHD3 一抗
  • 描述
    兔多克隆抗体to EHD3
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 30-60 of Human EHD3.

  • 阳性对照
    • Mouse lung tissue lysate. Brain tissue. IF/ICC: HepG2 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明ab103869 is antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB 1/50 - 1/100. Predicted molecular weight: 62 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性EHD3 contains one EF-hand domain and one EH domain. It plays a role in endocytic transport. EHD3 is highly expressed in heart and brain and moderately expressed in kidney, liver and placenta.
  • 细胞定位Endosome membrane, cytoplasmic side. Nucleus.
  • 数据库链接
  • 别名
    • EH domain containing 3 antibody
    • EHD2 antibody
    • EHD3 antibody
    • PAST3 antibody

Anti-EHD3 antibody 图像

  • ab103869 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103869 at 1/200 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-EHD3 antibody (ab103869) at 1/50 dilution + Mouse lung tissue lysate at 35 µg

    Predicted band size : 62 kDa
  • ab103869, at 1/50, staining EHD3 by immunohistochemistry in brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-EHD3 antibody (ab103869)参考文献

This product has been referenced in:

See 1 Publication for this product

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I am glad I could help you pick an integrin beta 3 antibody.

The only western blot data for human samples that we are aware of for antibody ab103869, anti-EDH3, are in the reference listed on the datasheet. Abcam has not tested ab103869 for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"