概述

  • 产品名称
  • 描述
    山羊多克隆抗体to EHD2
  • 特异性
    This antibody is expected to recognise EHD1 protein as well as EHD2.
  • 经测试应用
    适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-RLVPPSKRRHKGSA

    , corresponding to C terminal amino acids 529 - 542 of Human EHD2.

  • 阳性对照
    • Human heart lysate. Lysate prepared from transfected HEK293 transiently expressing EHD2. IF/ICC: A549 cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 20008164
ELISA 1/64000. Peptide ELISA
WB Use a concentration of 0.003 - 0.01 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能
    Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
  • 组织特异性
    Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.
  • 序列相似性
    Contains 1 EF-hand domain.
    Contains 1 EH domain.
  • 结构域
    The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
  • 细胞定位
    Cell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EH domain containing 2 antibody
    • EH domain containing 3 antibody
    • EH domain containing protein 2 antibody
    • EH domain-containing protein 2 antibody
    • EHD 2 antibody
    • Ehd2 antibody
    • EHD2_HUMAN antibody
    • EHD3 antibody
    • FLJ96617 antibody
    • PAST 2 antibody
    • PAST homolog 2 antibody
    • PAST2 antibody
    see all

图片

  • ICC/IF image of ab23935 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA  / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23935, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-EHD2 antibody (ab23935) at 0.003 µg/ml + Human Heart lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Anti-EHD2 antibody (ab23935) at 0.1 µg/ml + Human Placenta lysate in RIPA buffer at 35 µg

    Predicted band size : 61 kDa

文献

This product has been referenced in:

See all 10 Publications for this product

客户评价及客户问答

We have not tested this antibody with hamster, only human and mouse. The immunogen used to raise this antibody is RLVPPSKRRHKGSA and is 100% homologous with Chinese Hamster EDH1 (note that this antibody should recognize both EDH1 and EDH2).  Therefore,...

Read More
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
300 µg
Specification
HeLa
Immuno-precipitation step
Protein A
Username

Debora Rapaport

Verified customer

提交于 Feb 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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