重组
RabMAb

Anti-EGR2抗体[EPR4004] (ab108399)

概述

  • 产品名称
    Anti-EGR2抗体[EPR4004]
    参阅全部 EGR2 一抗
  • 描述
    兔单克隆抗体[EPR4004] to EGR2
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human EGR2.

  • 阳性对照
    • LnCaP, HepG2, MCF7, and SH SY5Y cell lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
  • 应用说明
    Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能
      Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.
    • 疾病相关
      Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot-Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
      Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D) [MIM:607678]. CMT1D is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    • 序列相似性
      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • 翻译后修饰
      Ubiquitinated by WWP2 leading to proteasomal degradation.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AT591 antibody
      • CMT1D antibody
      • CMT4E antibody
      • DKFZp686J1957 antibody
      • E3 SUMO-protein ligase EGR2 antibody
      • Early growth response 2 antibody
      • Early growth response protein 2 antibody
      • EGR-2 antibody
      • egr2 antibody
      • EGR2_HUMAN antibody
      • FLJ14547 antibody
      • KROX 20 Drosophila homolog antibody
      • Krox 20 homolog Drosophila antibody
      • KROX-20, Drosophila, homolog (early growth response-2) antibody
      • KROX20 antibody
      • Krox20 protein antibody
      • Zinc finger protein Krox-20 antibody
      see all

    Anti-EGR2 antibody [EPR4004] 图像

    • All lanes : Anti-EGR2 antibody [EPR4004] (ab108399) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : SH SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 53 kDa

    Anti-EGR2 antibody [EPR4004] (ab108399)参考文献

    This product has been referenced in:
    • Collombet S  et al. Logical modeling of lymphoid and myeloid cell specification and transdifferentiation. Proc Natl Acad Sci U S A 114:5792-5799 (2017). WB . Read more (PubMed: 28584084) »
    • Zhuo W  et al. MicroRNA-20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing early growth response 2 expression. Mol Med Rep 12:4989-94 (2015). WB . Read more (PubMed: 26238942) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - Triton X-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 06 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 03 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Specification
    U2OS cells
    Treatment
    50 pM TGF-beta time course
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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