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RabMAb

Anti-Egr1抗体[EPR5014(2)] (ab133695)

概述

  • 产品名称
    Anti-Egr1抗体[EPR5014(2)]
    参阅全部 Egr1 一抗
  • 描述
    兔单克隆抗体[EPR5014(2)] to Egr1
  • 经测试应用
    适用于: WB, Flow Cytmore details
    不适用于: ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human, Zebra finch
  • 免疫原

    corresponding to Human Egr1 aa 1-100 (internal sequence).
    Database link: P18146

  • 阳性对照
    • WB: Transfected 293T cell lysate Flow cyt: NIH/3T3 cells
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab133695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 57 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC/IF,IHC-P or IP.
  • 靶标

    • 功能
      Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
    • 序列相似性
      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AT225 antibody
      • Early growth response 1 antibody
      • Early growth response protein 1 antibody
      • EGR 1 antibody
      • EGR-1 antibody
      • EGR1 antibody
      • EGR1_HUMAN antibody
      • G0S30 antibody
      • KROX 24 antibody
      • KROX24 antibody
      • Nerve growth factor-induced clone A antibody
      • Nerve growth factor-induced protein A antibody
      • NGFI-A antibody
      • NGFIA antibody
      • TIS8 antibody
      • Transcription factor ETR103 antibody
      • Transcription factor Zif268 antibody
      • ZIF 268 antibody
      • ZIF268 antibody
      • Zinc finger protein 225 antibody
      • Zinc finger protein Krox-24 antibody
      • ZNF225 antibody
      see all

    图片

    • All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/1000 dilution

      Lane 1 : untreated 293 cell lysate
      Lane 2 : 293 cell lysate treated with 20% FBS

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • ab133695 (red), at 1/10, staining Egr1 in permeabilized NIH/3T3 cells treated with 20% FBS using flow cytometric analysis. The green curve corresponds to a Rabbit IgG (negative control).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    ab133695 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (7.5% Tris Glycine)
    Sample
    Zebra finch Cell lysate - whole cell (Hek cells overexpressing zebra finch EGR1)
    Specification
    Hek cells overexpressing zebra finch EGR1
    Treatment
    MG132
    Blocking step
    Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 28 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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