概述

  • 产品名称Anti-Egr1抗体
    参阅全部 Egr1 一抗
  • 描述
    小鼠单克隆抗体to Egr1
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: SPVATSYPSP VTTSYPSPAT TSYPSPVPTS FSSPGSSTYP SPVHSGFPSP SVATTYSSVP PAFPAQVSSF PSSAVTNSFS ASTGLSDMTA TFSPRTIEIC , corresponding to amino acids 444-544 of Human Egr1

性能

应用

Our Abpromise guarantee covers the use of ab54966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
  • 序列相似性Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AT225 antibody
    • Early growth response 1 antibody
    • Early growth response protein 1 antibody
    • EGR 1 antibody
    • EGR-1 antibody
    • EGR1 antibody
    • EGR1_HUMAN antibody
    • G0S30 antibody
    • KROX 24 antibody
    • KROX24 antibody
    • Nerve growth factor-induced clone A antibody
    • Nerve growth factor-induced protein A antibody
    • NGFI-A antibody
    • NGFIA antibody
    • TIS8 antibody
    • Transcription factor ETR103 antibody
    • Transcription factor Zif268 antibody
    • ZIF 268 antibody
    • ZIF268 antibody
    • Zinc finger protein 225 antibody
    • Zinc finger protein Krox-24 antibody
    • ZNF225 antibody
    see all

Anti-Egr1 antibody 图像

  • All lanes : Anti-Egr1 antibody (ab54966)

    Lane 1 : EGR1 transfected lysate
    Lane 2 : non-transfected


    Predicted band size : 58 kDa
  • Egr1 antibody (ab54966) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
  • Overlay histogram showing HeLa cells stained with ab54966 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54966, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Egr1 antibody (ab54966)参考文献

This product has been referenced in:
  • Bakalash S  et al. Egr1 expression is induced following glatiramer acetate immunotherapy in rodent models of glaucoma and Alzheimer's disease. Invest Ophthalmol Vis Sci 52:9033-46 (2011). Read more (PubMed: 21969301) »
  • Bieging KT  et al. A shared gene expression signature in mouse models of EBV-associated and non-EBV-associated Burkitt lymphoma. Blood 118:6849-59 (2011). WB . Read more (PubMed: 22039254) »

See all 3 Publications for this product

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