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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EGFR. Synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of mature human EGFR.
Database link: P00533
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab32430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 135 kDa.
A blocking peptide is available for this antibody as ab205609.
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/Immunofluorescence analysis of untreated, EGF treated and EGF + LP treated A431 cells labelling EGFR (phospho Y1068) with ab32430 (left) and EGFR with ab52894 (right) both at a dilution of 1/1000.
Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
The image shows increased membrane staining after EGF (100ng/ml, 30min) treatment on A431 cells. The LP treatment decreased the increased membrane staining caused by EGF.
ab52894 was used as a Pan control for ab32430. The results showed membrane and cytoplasmic staining on untreated, EGF and EGF + LP treated A431 cells.
Blocking and diluting buffer: 5% NFDM/TBST.
EGFR pY1068: 5 seconds.
EGFR: 1 minute.
GAPDH: 5 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.