Customer reviews and FAQs

概述

  • 产品名称
    Anti-EGFR抗体[EP38Y]
    参阅全部 EGFR 一抗
  • 描述
    兔单克隆抗体[EP38Y] to EGFR
  • 特异性
    The immunogen for this product is a synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR. After screening, clone “EP38Y” was found to recognize total EGFR and is not specific to phosphorylated-Tyr1068 EGFR. This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
  • 经测试应用
    适用于: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EGFR. Synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of mature human EGFR.
    Database link: P00533

  • 阳性对照
    • A431, HeLa cell lysate or human lymph node metastasis tissue.
  • 常规说明

    A trial size is available to purchase for this antibody.

    Alternative versions available:
    Anti-EGFR antibody (Alexa Fluor® 488) [EP38Y] (ab193244)
    Anti-EGFR antibody (Alexa Fluor® 647) [EP38Y] (ab192982)
    Anti-EGFR antibody (HRP) [EP38Y] (ab193602)
    Anti-EGFR antibody (Alexa Fluor® 594) [EP38Y] (ab207870)
    Anti-EGFR antibody (Phycoerythrin) [EP38Y] (ab208753)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab52894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 134 kDa).Can be blocked with EGFR peptide (ab204282).

This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR
IP 1/20.

For unpurified use at 1/50.
Flow Cyt 1/20.

For unpurified use at 1/100. 

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
ICC/IF 1/250 - 1/500.

靶标

  • 功能
    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 组织特异性
    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 疾病相关
    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • 细胞定位
    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody [EP38Y] 图像

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EP38Y] (ab52894)

    Unpurified ab52894 stained A431 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52894 at 1in500) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Anti-EGFR antibody [EP38Y] (ab52894) at 1/2000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 134 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)


    Predicted band size : 134 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    ab52894 (purified) at 1:20 dilution (0.5ug) immunoprecipitating EGFR in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2 (+): ab52894 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52894 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    All lanes : Anti-EGFR antibody [EP38Y] (ab52894) at 1/10000 dilution (purified)

    Lane 1 : Rat liver lysates
    Lane 2 : Mouse lung lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 134 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    All lanes : Anti-EGFR antibody [EP38Y] (ab52894) at 1/1000 dilution (unpurified)

    Lane 1 : Caco-2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Mouse skin cell lysate
    Lane 4 : Rat skin cell lysate

    Lysates/proteins at 20 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 134 kDa

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with unpurified ab52894 overnight at 4°C in the presence of loading control ab18058 (Mouse monoclonal [SPM227] to Vinculin diluted 1:10000). Antibody binding was detected using IR-labelled goat anti-Rabbit Ab at a 1:10,000 dilution for one hour at room temperature before imaging.

    This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein.  Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR.

  • Flow Cytometry - Anti-EGFR antibody [EP38Y] (ab52894)
    Flow Cytometry - Anti-EGFR antibody [EP38Y] (ab52894)

    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab52894 at 1:20 dilution (5 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black).Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EP38Y] (ab52894)

    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling EGFR with Purified ab52894 at 1:250 dilution (0.4μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling EGFR with purified ab52894 at 1:100 dilution (0.95 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)

    Unpurified ab52894 showing positive staining in Endometrial carcinoma tissue.

  • Flow Cytometry - Anti-EGFR antibody [EP38Y] (ab52894)
    Flow Cytometry - Anti-EGFR antibody [EP38Y] (ab52894)

    Overlay histogram showing A431 cells stained with unpurified ab52894 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52894, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Anti-EGFR antibody [EP38Y] (ab52894) at 1/100000 dilution (unpurified) + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size : 134 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)

    Unpurified ab52894 showing positive staining in Cervical carcinoma tissue.

  • Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)Image is courtesy of an anonymous AbReview.
    Anti-EGFR antibody [EP38Y] (ab52894) at 1/500 dilution (unpurified) + Murine macrophage lysates at 80 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Additional bands at : 170 kDa (possible post-translational modification).

    Exposure time : 1 minute

    Image is courtesy of an anonymous AbReview.

    Blocking was with 5% BSA incubated for 1 hour at 25°C.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)

    Unpurified ab52894 showing positive staining in Glioma tissue.

  • Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
    All lanes : Anti-EGFR antibody [EP38Y] (ab52894) at 1/1000 dilution (unpurified)

    Lane 1 : H820 siCtl cell lysate, siRNA treated
    Lane 2 : H820 siEGFR#1 cell lysate, siRNA treated
    Lane 3 : H820 siEGFR#2 cell lysate, siRNA treated

    Lysates/proteins at 20 µg per lane.

    Secondary
    GE Healthcare Polyclonal Anti Rabbit, HRP conjugated at 1/5000 dilution

    Predicted band size : 134 kDa
    Observed band size : 165 kDa (why is the actual band size different from the predicted?)

    Gel running conditions: 4-15%, reduced and denatured.

    Blocking agent: 5% milk.

    Blocking time: 1 hour.

    Incubation (with primary antibody): 16 hours, 4 Celsius.

    Dilution buffer: 5%BSA

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)

    Unpurified ab52894 showing positive staining in Normal tonsil squamous cells tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (ab52894)

    Unpurified ab52894 showing positive staining in Renal cell carcinoma tissue.

  • Other - Anti-EGFR antibody [EP38Y] (ab52894)
    Other - Anti-EGFR antibody [EP38Y] (ab52894)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-EGFR antibody [EP38Y] (ab52894)参考文献

This product has been referenced in:
  • Chen CC  et al. The matricellular protein CCN1 suppresses hepatocarcinogenesis by inhibiting compensatory proliferation. Oncogene 35:1314-23 (2016). WB . Read more (PubMed: 26028023) »
  • Wang G  et al. Simvastatin induces cell cycle arrest and inhibits proliferation of bladder cancer cells via PPAR? signalling pathway. Sci Rep 6:35783 (2016). WB . Read more (PubMed: 27779188) »

See all 19 Publications for this product

发表研究结果有使用 ab52894？请让我们知道，以便我们可以引用本数据表中的参考文章。

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10 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-EGFR antibody [EP38Y]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Treatment
Unilateral Ureter Obstruction Surgery
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Apr 05 2017

Western blot abreview for Anti-EGFR antibody [EP38Y]

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Renal Epithelial Cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (8%)
Loading amount
60 µg
Treatment
20ng EGF for 30min
Specification
Human Renal Epithelial Cells
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Dr. 腾飞 白

Verified customer

提交于 Jan 06 2016

Western blot abreview for Anti-EGFR antibody [EP38Y]

 Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
80 µg
Specification
macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

提交于 Nov 06 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-EGFR antibody [EP38Y]

 Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (D3 mouse embryonic stem cells)
Permeabilization
Yes - Triton 0.1%
Specification
D3 mouse embryonic stem cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
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Miss. Fabiana Luise

Verified customer

提交于 Oct 22 2015

Western blot abreview for Anti-EGFR antibody [EP38Y]

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Human Cell lysate - whole cell (non small cell lung cancer)
Specification
non small cell lung cancer
Treatment
siRNA treated
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

提交于 Oct 07 2014




Thank you for all the advice and the help you gave me

Please I was reading the protocol of the IHC kit I bought and I would like to have more information about the 3rd step in the staining protocol when you speak about perfoming...

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The pretreatment note in the protocol of the IHC kit refers to the antigen retrieval step that is required for most antibody to achieve a successful IHC-P staining.
For both of these antibodies (ab14440 and ab52894) I can recommend to use Sod...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-EGFR antibody [EP38Y]

 Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
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Username

Abcam user community

Verified customer

提交于 Mar 25 2014

Dear Technical support,
I am Suping working in UCSD and is looking for EGFR antibody for IHC-P. I did find some EGFR antibodies (e.g.ab52894). However, it does not indicate if need unmasking procedure and what method for this step. Would you pleas...

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Hello! We do recommend you use an antigen retrieval step, heat mediated such as citrate pH6. The antibody you suggest, ab52894 is a good one. Another EGFR antibody with good feedback is ab9482. We do guarantee our products to work as specified on the d...

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Hi,   Can you please let me know the concentration of this antibody? Anti-EGFR antibody [EP38Y] (ab52894). I am looking for an EGFR monoclonal antibody which concentration is at least or higher than 1mg/ml.  Do you have anyone match these criteria? ...

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Thank you for contacting us.

Rabbit monoclonals typically have 10-100x the affinity of mouse monoclonals, as such it is often the case that the concentration of a rabbit monoclonal will be lower than a mouse monoclonal. Ab52894 does not ha...

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I'm using this antibody and I was trying to find some information on the stability of the antigen once a FFPE tissue has been sectioned. I did not see any info on the product specification sheet. Do you have this information?

Paraffin blocks are commonly cut into 4 µm thickness sections and those sections are stored in a sealed box at room temperature for no more than 6 months. We have not observed any particular rapid degradation of the EGFR antigen.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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