概述

  • 产品名称Anti-EGFR抗体
    参阅全部 EGFR 一抗
  • 描述
    兔多克隆抗体to EGFR
  • 特异性ab15669 specifically detects EGFR protein in WB and IHC-Fr in mouse brain. In WB, ab15669 detects a larger MW band (200kDa) than predicted (134kDa); this is likely to be the glycosylated form of EGFR protein.
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from residues 350 - 450 of Mouse EGFR.

    .

  • 阳性对照
    • This antibody gave a positive signal in Mouse Brain Tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab15669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 134 kDa).
IHC-Fr Use a concentration of 2 - 5 µg/ml.

靶标

  • 功能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 组织特异性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 疾病相关Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • 细胞定位Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt
  • 数据库链接
  • 别名
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR antibody 图像

  • ab15669 EGFR antibody staining in E17 mouse cerebral cortex (5ug/ml). E17 mouse embryos were fixed in 4% paraformaldehyde in PB overnight at 4°C then transferred to 30% sucrose in PBS. Brains were frozen in TissueTek and stored -80°C. Cryostat coronal sections were cut at 12um. Sections were blocked in 0.1% triton in PBS containing 10% normal serum from the species that secondary antibody was generated from. Primary antibodies were incubated on the sections at 4°C overnight. Secondary antibodies were incubated for 2 hours at RT. Images were taken using a Zeiss camera under 40X objectives

Anti-EGFR antibody (ab15669)参考文献

This product has been referenced in:
  • Al-Nedawi K  et al. Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR. Proc Natl Acad Sci U S A 106:3794-9 (2009). IHC-P ; Mouse . Read more (PubMed: 19234131) »
  • Alagappan D  et al. Brain injury expands the numbers of neural stem cells and progenitors in the SVZ by enhancing their responsiveness to EGF. ASN Neuro 1: (2009). WB ; Rat . Read more (PubMed: 19570028) »

See all 3 Publications for this product

Product Wall

Thank you for your reply.

I appreciate the time that you took to try and optimize the protocol using ab2430. I am sorry to learn that this different fixation did not improve the staining.

As requested, I have issued a free of charge...

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Thank you for contacting us.

We have 46 polyclonal EGFR antibodies in our catalog, three of which are raised against the extracellular domain (residues 25 - 645):

ab15669 (http://www.abcam.com/EGFR-antibody-ab15669.html)
ab103...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"