重组Anti-EGF抗体[EPR19174] (ab184266)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19174] to EGF
- Suitable for: WB, IHC-Fr, IP
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-EGF抗体[EPR19174]
参阅全部 EGF 一抗 -
描述
兔单克隆抗体[EPR19174] to EGF -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Fr, IPmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse EGF recombinant protein; Mouse salivary gland and kidney lysates; Mouse urine sample. IHC-Fr: Mouse kidney tissue. IP: Mouse urine sample
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19174 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184266于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 160, 6 kDa (predicted molecular weight: 133 kDa).
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IHC-Fr |
1/500.
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IP |
1/40.
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说明 |
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WB
1/1000. Detects a band of approximately 160, 6 kDa (predicted molecular weight: 133 kDa). |
IHC-Fr
1/500. |
IP
1/40. |
靶标
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功能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
组织特异性
Expressed in kidney, salivary gland, cerebrum and prostate. -
疾病相关
Hypomagnesemia 4 -
序列相似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻译后修饰
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13645 Mouse
- SwissProt: P01132 Mouse
- Unigene: 252481 Mouse
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别名
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
图片
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on mouse kidney is observed. The staining pattern is consistent with what has been described in the literature (PMID:17671655).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).
Weak staining on mouse liver is observed. The expression profile observed is consistent with what has been described in the literature (PMID:17671655).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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Anti-EGF antibody [EPR19174] (ab184266) at 1/2000 dilution + Mouse EGF recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 6 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-EGF antibody [EPR19174] (ab184266) at 1/1000 dilution + Mouse salivary gland lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 6 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:2268351 & 7499272).
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All lanes : Anti-EGF antibody [EPR19174] (ab184266) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse urine sample
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 8 seconds; Lane 2: 5 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:2268351 & 7499272).
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EGF was immunoprecipitated from 0.35 mg of Mouse urine sample with ab184266 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184266 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse urine sample, 10µg (Input).
Lane 2: ab184266 IP in Mouse urine sample.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184266 in Mouse urine sample.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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