Anti-EGF抗体[EGF-10] (ab10409)

概述

  • 产品名称Anti-EGF抗体[EGF-10]
    参阅全部 EGF 一抗
  • 描述
    小鼠单克隆抗体[EGF-10] to EGF
  • 特异性Ab10409 was tested against recombinant EGF, Abcam therefore cannot guarantee it will be suitable against endogenous EGF in WB.
  • 经测试应用适用于: Dot blot, IHC-Fr, ELISA, RIA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant full length protein (Human).

  • 常规说明If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab10409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use a concentration of 0.1 µg/ml.
IHC-Fr 1/500. PubMed: 17475821(see Abreview)
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-P 1/500.

靶标

  • 功能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 组织特异性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 疾病相关Hypomagnesemia 4
  • 序列相似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻译后修饰O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Anti-EGF antibody [EGF-10] 图像

  • ab10409 staining EGF in human normal skin tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde. Samples were blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/500 dilution for 30 minutes at 21°C. An undiluted HRP-conjugated goat polyclonal was used as the secondary antibody.

    See Abreview

Anti-EGF antibody [EGF-10] (ab10409)参考文献

This product has been referenced in:
  • Lindenmeyer MT  et al. Interstitial vascular rarefaction and reduced VEGF-A expression in human diabetic nephropathy. J Am Soc Nephrol 18:1765-76 (2007). IHC-P, IHC-Fr ; Human . Read more (PubMed: 17475821) »

See 1 Publication for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for contacting Abcam regarding ab10409. I have confirmed the following protocol was used in IHC-P: Heat Induced Epitope Retrieval (HIER) is performed by heating the slides immersed in retrieval solution: 10 mM sodium citrate buffer, p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Normal Skin)
Specification Normal Skin
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jan 11 2011

Thank you for your enquiry. We tested ab10409 with a recombinant EGF sample, which is available from Sigma I believe, catalogue number E9644. Please do not hesitate to contact me again if you require further information,

Thanks for your enquiry regarding ab10409. Unfortunately we do not currently offer free trial size batches of antibodies to test. I should take this opportunity to mention that this antibody may not recognise rat EGF since the antibody is known not...

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Thank you for your note. We are very sorry to hear that you are still struggling with this antibody and even the replacement vial does not perform as it should do. We can offer you either a credit note or a full refund; please do let us know how to...

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Thank you for your enquiry and forwarding your detailed protocol to us. We are very sorry to hear that you are having problem with this antibody. We would suggest loading purified human EGF (1-100 ng) as positive control along with the samples. ...

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We are not aware of any publications where this antibody is used. However, if you have a look at the reviews section of the online datasheet, you'll see that another Abcam customer used this antibody in IHC on formalin-fixed paraffin-embedded human aut...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"