Anti-EGF抗体(Biotin) (ab83520)

概述

  • 产品名称
    Anti-EGF抗体(Biotin)
    参阅全部 EGF 一抗
  • 描述
    山羊多克隆抗体to EGF (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: ELISA, Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mouse EGF

  • 常规说明
    ab83520 is sterile filtered.

性能

应用

Our Abpromise guarantee covers the use of ab83520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Sandwich ELISA
WB
  • 应用说明
    ELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse EGF.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse EGF.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant mouse EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 133 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
    • 组织特异性
      Expressed in kidney, salivary gland, cerebrum and prostate.
    • 疾病相关
      Hypomagnesemia 4
    • 序列相似性
      Contains 9 EGF-like domains.
      Contains 9 LDL-receptor class B repeats.
    • 翻译后修饰
      O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta urogastrone antibody
      • beta-urogastrone antibody
      • Egf antibody
      • EGF_HUMAN antibody
      • Epidermal growth factor antibody
      • HOMG4 antibody
      • OTTHUMP00000219721 antibody
      • OTTHUMP00000219722 antibody
      • Pro epidermal growth factor antibody
      • URG antibody
      • Urogastrone antibody
      see all

    实验方案

    文献

    ab83520 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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