概述

性能

应用

Our Abpromise guarantee covers the use of ab9695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml. To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa.
ELISA Use a concentration of 0.5 µg/ml. To detect hEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF.
Neutralising Use a concentration of 0.25 - 0.35 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab18635 as Capture.

IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能
    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 组织特异性
    Expressed in kidney, salivary gland, cerebrum and prostate.
  • 疾病相关
    Hypomagnesemia 4
  • 序列相似性
    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻译后修饰
    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta urogastrone antibody
    • beta-urogastrone antibody
    • Egf antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

图片

  • ab9695 at 1.0 µg/ml staining normal human skin by IHC-P. The antibody was incubated overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.
  • Primary ab9695 was used at a dilution of 0.2 ug/ml.

    Lane 1: 250 ng Recombinant human EGF protein

    Lane 2: 125 ng Recombinant human EGF protein

  • Immunohistochemical analysis of murine forepaw vessel, staining EGF with ab9695.

    Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (4 µg/ml in BSA in TBS) for 1 hour. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Standard Curve for EGF (Analyte: ab51082) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [S-177] to EGF (ab18635) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGF (ab9695) at 0.5ug/ml

文献

This product has been referenced in:
  • Zhang B  et al. Metastasis-associated protein 2 promotes the metastasis of non-small cell lung carcinoma by regulating the ERK/AKT and VEGF signaling pathways. Mol Med Rep 17:4899-4908 (2018). Read more (PubMed: 29393472) »
  • Fang C  et al. Berberine ameliorates neonatal necrotizing enterocolitis by activating the phosphoinositide 3-kinase/protein kinase B signaling pathway. Exp Ther Med 15:3530-3536 (2018). Read more (PubMed: 29545879) »

See all 11 Publications for this product

客户评价及客户问答

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Abreviews
Application
Western blot
Sample
Human Recombinant protein (Human recombinant EGF)
Gel Running Conditions
Reduced Denaturing (16% tricine gel MES)
Loading amount
0.002 µg
Specification
Human recombinant EGF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

提交于 May 26 2015

Abreviews
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (4-10%)
Sample
Orthopoxvirus Serum (BHK-21 cells)
Specification
BHK-21 cells
Treatment
Infected with Western Reserve Strain of vaccinia virus
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Dr. B Ward

Verified customer

提交于 Oct 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Forepaw Vessel)
Specification
Forepaw Vessel
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Prot K
Permeabilization
No
Blocking step
DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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