Anti-EGF抗体(ab94898)
Key features and details
- Rabbit polyclonal to EGF
- Suitable for: ELISA, Indirect ELISA, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EGF抗体
参阅全部 EGF 一抗 -
描述
兔多克隆抗体to EGF -
宿主
Rabbit -
经测试应用
适用于: ELISA, Indirect ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant Human EGF
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab94898 was purified by affinity chromatography employing immobilized Human EGF matrix -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94898于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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Indirect ELISA |
Use at an assay dependent concentration.
To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 133 kDa.
Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions. |
说明 |
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ELISA
Use at an assay dependent concentration. |
Indirect ELISA
Use at an assay dependent concentration. To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 133 kDa. Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions. |
靶标
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功能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
组织特异性
Expressed in kidney, salivary gland, cerebrum and prostate. -
疾病相关
Hypomagnesemia 4 -
序列相似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻译后修饰
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1950 Human
- Omim: 131530 Human
- SwissProt: P01133 Human
- Unigene: 419815 Human
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别名
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
数据表及文件
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Datasheet download
文献 (0)
ab94898 尚未被引用在任何文献中。