概述

  • 产品名称Anti-EGF抗体
    参阅全部 EGF 一抗
  • 描述
    兔多克隆抗体to EGF
  • 经测试应用适用于: WB, Neutralising, Indirect ELISA, Sandwich ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Highly pure (>98%) recombinant Rat EGF.

性能

应用

Our Abpromise guarantee covers the use of ab77851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 6.2 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 0.1 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/ml).
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. In conjunction with compatible secondary reagents, ab77851 allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21121876
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 组织特异性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 疾病相关Hypomagnesemia 4
  • 序列相似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻译后修饰O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Anti-EGF antibody (ab77851)参考文献

This product has been referenced in:
  • Li H  et al. Different expression patterns of growth factors in rat fetuses with spina bifida aperta after in utero mesenchymal stromal cell transplantation. Cytotherapy 16:319-30 (2014). IHC-P ; Rat . Read more (PubMed: 24364908) »
  • Williams CM  et al. Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays. Tissue Eng Part A 17:1055-68 (2011). ICC/IF ; Rat . Read more (PubMed: 21121876) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"