生物素Anti-EGF抗体[3A8] (ab155576)
Key features and details
- Biotin Mouse monoclonal [3A8] to EGF
- Suitable for: ICC/IF, Sandwich ELISA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-EGF抗体[3A8]
参阅全部 EGF 一抗 -
描述
生物素小鼠单克隆抗体[3A8] to EGF -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: ICC/IF, Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant Human EGF protein (NP_001954.2).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 95% PBS, 4% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
3A8 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155576于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50.
|
|
Sandwich ELISA |
Use a concentration of 0.125 - 0.25 µg/ml.
|
说明 |
---|
ICC/IF
1/50. |
Sandwich ELISA
Use a concentration of 0.125 - 0.25 µg/ml. |
靶标
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功能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
组织特异性
Expressed in kidney, salivary gland, cerebrum and prostate. -
疾病相关
Hypomagnesemia 4 -
序列相似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻译后修饰
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1950 Human
- Omim: 131530 Human
- SwissProt: P01133 Human
- Unigene: 419815 Human
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别名
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
图片
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Sandwich ELISA analysis of Human EGF using ab155576.
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Immunocytochemical analysis of EGF (green) in HeLa cells untreated (left panel) or treated with 10 ng/mL EGF (right panel) for 5 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100. Cells were probed with ab155576 at a dilution of 1/50 for 1 hour at room temperature, followed by DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab155576 尚未被引用在任何文献中。