Anti-Eg5 (phospho T927)抗体(ab61104)


  • 产品名称Anti-Eg5 (phospho T927)抗体
    参阅全部 Eg5 一抗
  • 描述
    兔多克隆抗体to Eg5 (phospho T927)
  • 特异性ab61104 detects endogenous levels of Eg5 only when phosphorylated at threonine 927.
  • 经测试应用适用于: WB, ICC/IF, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of threonine 927 (G-T-TP-P-Q).

  • 阳性对照
    • Human lung carcinoma tissue, extracts from COLO205 cells.



Our Abpromise guarantee covers the use of ab61104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
  • 疾病相关Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
  • 序列相似性Belongs to the kinesin-like protein family. BimC subfamily.
    Contains 1 kinesin-motor domain.
  • 翻译后修饰Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
    A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EG5 antibody
    • HKSP antibody
    • KIF11 antibody
    • KIF11_HUMAN antibody
    • Kinesin family member 11 antibody
    • Kinesin like protein 1 antibody
    • Kinesin-like protein 1 antibody
    • Kinesin-like protein KIF11 antibody
    • Kinesin-like spindle protein HKSP antibody
    • Kinesin-related motor protein Eg5 antibody
    • KNSL1 antibody
    • MCLMR antibody
    • Thyroid receptor-interacting protein 5 antibody
    • TR-interacting protein 5 antibody
    • TRIP-5 antibody
    • TRIP5 antibody
    see all

Anti-Eg5 (phospho T927) antibody 图像

  • All lanes : Anti-Eg5 (phospho T927) antibody (ab61104) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells.
    Lane 2 : Extracts from COLO205 cells with the immunising peptide

    Predicted band size : 120 kDa
    Observed band size : 120 kDa
  • ICC/IF image of ab61104 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61104, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab61104 staining in human bone marrow chronic lymphocytic leukemia formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61104, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Eg5 (phospho T927) antibody (ab61104)参考文献

ab61104 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblast Cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 25 µg
Treatment 150 nM nocodazole for 5.5 hrs
Specification Mouse Embryonic Fibroblast Cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 May 12 2016