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Synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Human Eg5.
(Peptide available as ab38094.)
Our Abpromise guarantee covers the use of ab51976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|