• 产品名称
  • 描述
    兔多克隆抗体to EFTUD2
  • 经测试应用
    适用于: IHC-P, ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide (Human) corresponding to a region between residues 1 and 50 of EFTUD2. (NP_004238.2)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.


  • 形式
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab72456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 109 kDa).
IP Use at 2-5 µg/mg of lysate.


  • 功能
    Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
  • 疾病相关
    Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM) [MIM:610536]. A rare syndrome characterized by progressive microcephaly, midface and malar hypoplasia, micrognathia, microtia, dysplastic ears, preauricular skin tags, significant developmental delay, and speech delay. Many patients have major sequelae, including choanal atresia that results in respiratory difficulties, conductive hearing loss, and cleft palate.
  • 序列相似性
    Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • 116 kDa antibody
    • 116 kDa U5 small nuclear ribonucleoprotein component antibody
    • EFTUD2 antibody
    • Elongation factor Tu GTP binding domain containing 2 antibody
    • Elongation factor Tu GTP-binding domain-containing protein 2 antibody
    • hSNU114 antibody
    • MFDGA antibody
    • MFDM antibody
    • SNRNP116 antibody
    • Snrp116 antibody
    • Snu114 antibody
    • SNU114 homolog antibody
    • U5 116KD antibody
    • U5 small nuclear ribonucleoprotein component antibody
    • U5 snRNP specific protein, 116 kD antibody
    • U5 snRNP specific protein, 116 kDa antibody
    • U5 snRNP-specific protein antibody
    • U5-116 kDa antibody
    • U5-116KD antibody
    • U5S1_HUMAN antibody
    see all

Anti-EFTUD2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling EFTUD2 with ab72456 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EFTUD2 antibody (ab72456) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size : 109 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
  • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab72456 used for IP at 3 µg/mg lysate, and at 0.1 µg/ml for subsequent WB.
    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • IHC image of ab72456 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72456, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab110240 staining EFTUD2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% donkey serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000 in PBS) for 1.5 hours at 25°C. A Cy3®-conjugated sheep anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody. DNA was stained with TO-PRO®-3.

    See Abreview

Anti-EFTUD2 antibody (ab72456)参考文献

ab72456 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - Triton 0.3%
Blocking step
Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 Jul 02 2012