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Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (ATDELATKLS RRLQMEGEGG GETPEQPGLN GAAAAAAGAP DEAAEALGSA) of Human EFHD2, NP_077305
Our Abpromise guarantee covers the use of ab87006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
ab87006 staining EFHD2 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in 10mM citrate pH6, 0.1% Tween 20. Samples were incubated with primary antibody (diluted in 0.1% TritonX-100 in PBS) for 16 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.