概述

  • 产品名称Anti-EFHD2抗体
    参阅全部 EFHD2 一抗
  • 描述
    山羊多克隆抗体to EFHD2
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human EFHD2 aa 230-240 (C terminal).
    Sequence:

    (C)AAFKELQSTFK

  • 阳性对照
    • WB: Human tonsil, human thymus, human lymph node and mouse thymus tissue lysates. IHC-P: Human tonsil tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 28-30 kDa.

靶标

  • 功能May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
  • 组织特异性Found in lymphocytes; preferentially expressed in CD8+ cells.
  • 序列相似性Contains 2 EF-hand domains.
  • 细胞定位Membrane raft. In immature B-cell line WEHI231.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EF hand domain containing protein 2 antibody
    • EF hand domain family, member D2 antibody
    • EF-hand domain-containing protein D2 antibody
    • EFHD 2 antibody
    • EFHD2 antibody
    • EFHD2_HUMAN antibody
    • RP3-467K16.3 antibody
    • Swiprosin 1 antibody
    • Swiprosin-1 antibody
    • SWS1 antibody
    see all

Anti-EFHD2 antibody 图像

  • All lanes : Anti-EFHD2 antibody (ab24368) at 0.3 µg/ml

    Lane 1 : Human tonsil lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human lymph node lysate
    Lane 4 : Mouse thymus lysate

    Lysates/proteins at 35 µg per lane.
  • Immunohistochemistry (Paraffin-embedded sections) analysis of paraffin embedded human tonsil tissue labeling EFHD2 with ab24368 at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

  • ab24368 staining EFHD2 in Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 5% serum) for 1 hour. DyLight® 594-conjugated Mouse anti-goat IgG polyclonal (7µg/ml) was used as the secondary antibody.

    See Abreview

Anti-EFHD2 antibody (ab24368)参考文献

This product has been referenced in:
  • Borger E  et al. The calcium-binding protein EFhd2 modulates synapse formation in vitro and is linked to human dementia. J Neuropathol Exp Neurol 73:1166-82 (2014). WB . Read more (PubMed: 25383639) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Neuroblastoma)
Specification Neuroblastoma
Permeabilization Yes - 0.1% Tx100, 5min
Fixative Formaldehyde
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提交于 May 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"