概述

  • 产品名称Anti-EFHA1抗体
    参阅全部 EFHA1 一抗
  • 描述
    兔多克隆抗体to EFHA1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 95-238 of Human EFHA1 (AAH31089).

  • 阳性对照
    • U87-MG whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab101465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.

靶标

Anti-EFHA1 antibody 图像

  • Anti-EFHA1 antibody (ab101465) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size : 50 kDa

Anti-EFHA1 antibody (ab101465)参考文献

This product has been referenced in:
  • Lee Y  et al. Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter. EMBO Rep N/A:N/A (2015). WB ; Human . Read more (PubMed: 26341627) »
  • Plovanich M  et al. MICU2, a paralog of MICU1, resides within the mitochondrial uniporter complex to regulate calcium handling. PLoS One 8:e55785 (2013). WB ; Human . Read more (PubMed: 23409044) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 25 µg
Specification Prostate cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 04 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (heart)
Specification heart
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Giulia Borile

Verified customer

提交于 Jun 23 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (4-12% BIS-TRIS)
Sample Human Cell lysate - whole cell (fibroblasts)
Specification fibroblasts
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"