概述
- 产品名称Anti-EEF2K (phospho S366)抗体
参阅全部 EEF2K 一抗 - 描述兔多克隆抗体to EEF2K (phospho S366)
- 特异性This antibody detects endogenous levels of EEF2K only when phosphorylated at serine 366.
- 经测试应用适用于: IHC-P, WB, ELISAmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat
- 免疫原
A synthesized phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).
- 阳性对照
- Hela cells.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab51227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50 - 1/100. | |
| WB | 1/300 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). | |
| ELISA | 1/40000. |
靶标
- 功能Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
- 序列相似性Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: O00418 Human
- SwissProt: O08796 Mouse
- SwissProt: P70531 Rat
- Unigene: 498892 Human
see all -
别名
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
Anti-EEF2K (phospho S366) antibody 图像
-
Western blot - EEF2K (phospho S366) antibody (ab51227)All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution
Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.
Predicted band size : 95 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S366) antibody (ab51227)Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)
Anti-EEF2K (phospho S366) antibody (ab51227)参考文献
ab51227 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"