Anti-EEF2K (phospho S359)抗体(ab111511)

概述

  • 产品名称Anti-EEF2K (phospho S359)抗体
    参阅全部 EEF2K 一抗
  • 描述
    兔多克隆抗体to EEF2K (phospho S359)
  • 特异性ab111511 detects endogenous levels of EEF2K only when phosphorylated at serine 359 (Human: Ser359; Mouse: Ser358; Rat: Ser358).
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human EEF2K around the phosphorylation site of serine 359 (G-SP-P-R-V).

  • 阳性对照
    • Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111511 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
  • 序列相似性Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
    Contains 1 alpha-type protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C86191 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
    • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody
    • Calmodulin dependent protein kinase III antibody
    • cb365 antibody
    • EC 2.7.11.20 antibody
    • eEF 2 kinase antibody
    • eEF 2K antibody
    • eEF-2 kinase antibody
    • eEF-2K antibody
    • Eef2k antibody
    • EEF2K protein antibody
    • EF2K_HUMAN antibody
    • Elongation factor 2 kinase antibody
    • Eukaryotic elongation factor 2 kinase antibody
    • fa04b08 antibody
    • HSU93850 antibody
    • kinase eEF2K antibody
    • MGC45041 antibody
    • SMEF2K antibody
    • wu:fa04b08 antibody
    • zgc:100973 antibody
    see all

Anti-EEF2K (phospho S359) antibody 图像

  • ab111511, at 1/50 dilution, staining EEF2K in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-EEF2K (phospho S359) antibody (ab111511)参考文献

ab111511 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Specification colon
Blocking step background sniper as blocking agent for 8 minute(s) · Concentration: 100%
Fixative formalin
Username

Ms. Cheri Pasch

Verified customer

提交于 Jul 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"