概述

  • 产品名称Anti-EEF2K抗体[EP881Y]
    参阅全部 EEF2K 一抗
  • 描述
    兔单克隆抗体[EP881Y] to EEF2K
  • 经测试应用适用于: WB, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminal of human EEF2K.

  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

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应用

Our Abpromise guarantee covers the use of ab45168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 82 kDa.
ICC/IF 1/50 - 1/100.
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
    • 序列相似性Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
      Contains 1 alpha-type protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C86191 antibody
      • Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
      • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
      • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody
      • Calmodulin dependent protein kinase III antibody
      • cb365 antibody
      • EC 2.7.11.20 antibody
      • eEF 2 kinase antibody
      • eEF 2K antibody
      • eEF-2 kinase antibody
      • eEF-2K antibody
      • Eef2k antibody
      • EEF2K protein antibody
      • EF2K_HUMAN antibody
      • Elongation factor 2 kinase antibody
      • Eukaryotic elongation factor 2 kinase antibody
      • fa04b08 antibody
      • HSU93850 antibody
      • kinase eEF2K antibody
      • MGC45041 antibody
      • SMEF2K antibody
      • wu:fa04b08 antibody
      • zgc:100973 antibody
      see all

    Anti-EEF2K antibody [EP881Y] 图像



    • Predicted band size : 82 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: EEF2K knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab45168 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab45168 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab45168 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution + HEK293 cell lysate

      Predicted band size : 82 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)

    Anti-EEF2K antibody [EP881Y] (ab45168)参考文献

    This product has been referenced in:
    • Tavares CD  et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem 289:23901-16 (2014). Human . Read more (PubMed: 25012662) »

    See 1 Publication for this product

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