Anti-EEF2K抗体(ab96685)
Key features and details
- Rabbit polyclonal to EEF2K
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-EEF2K抗体
参阅全部 EEF2K 一抗 -
描述
兔多克隆抗体to EEF2K -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human EEF2K aa 1-238.
Database link: O00418 -
阳性对照
- ICC/IF: A431 cells. IHC-P: BT483 xenograft. WB: A431 , H1299 , HeLa , HepG2 cells. Mouse liver lysate. PC-12 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/10000. Predicted molecular weight: 82 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/10000. Predicted molecular weight: 82 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin. -
序列相似性
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: O00418 Human
- SwissProt: O08796 Mouse
- SwissProt: P70531 Rat
- Unigene: 498892 Human
see all -
别名
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
图片
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Paraffin embedded Mouse BT483 xenograft tissue stained for eEF-2K using ab96685 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-EEF2K antibody (ab96685) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 82 kDa
7.5% SDS-PAGE -
ab96685, at 1/200 dilution staining EEF2K in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
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Anti-EEF2K antibody (ab96685) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 82 kDa7.5% SDS PAGE
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Anti-EEF2K antibody (ab96685) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Predicted band size: 82 kDa7.5% SDS-PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab96685 被引用在 1 文献中.
- Chen X et al. eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma. J Immunother Cancer 10:N/A (2022). PubMed: 35347072