RabMAb

Anti-EEF2抗体[EP723Y] (ab40762)

概述

  • 产品名称Anti-EEF2抗体[EP723Y]
    参阅全部 EEF2 一抗
  • 描述
    兔单克隆抗体[EP723Y] to EEF2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human eEF2 was used as immunogen.

  • 阳性对照
    • 293 cell lysate, human lymphoma (IHC-P).
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP723Y
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab40762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/50.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
    • 序列相似性Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
    • 翻译后修饰Phosphorylation by EF-2 kinase completely inactivates EF-2.
      Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
      ISGylated.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EEF 2 antibody
      • Eef2 antibody
      • EF-2 antibody
      • EF2 antibody
      • EF2_HUMAN antibody
      • Elongation factor 2 antibody
      • Eukaryotic translation elongation factor 2 antibody
      • Polypeptidyl tRNA translocase antibody
      • SCA26 antibody
      see all

    Anti-EEF2 antibody [EP723Y] 图像

    • Anti-EEF2 antibody [EP723Y] (ab40762) at 1/1000 dilution + HEK293 cell lysate at 10 µg

      Predicted band size : 95 kDa
      Observed band size : 95 kDa
    • ab40762 (1/50) staining human lymphoma by immunohistochemistry, paraffin embedded tissue.

    Anti-EEF2 antibody [EP723Y] (ab40762)参考文献

    ab40762 has not yet been referenced specifically in any publications.

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