概述

  • 产品名称Anti-EEF2抗体
    参阅全部 EEF2 一抗
  • 描述
    兔多克隆抗体to EEF2
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Carp, Snake, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 808 - 858 of Human EEF2 (NP_001952.1)

  • 阳性对照
    • Human: breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, skin melanoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: colon carcinoma CT26, teratoma.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may enhance staining.
    Note: expected to work on frozen sections.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
    • 序列相似性Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
    • 翻译后修饰Phosphorylation by EF-2 kinase completely inactivates EF-2.
      Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
      ISGylated.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EEF 2 antibody
      • Eef2 antibody
      • EF-2 antibody
      • EF2 antibody
      • EF2_HUMAN antibody
      • Elongation factor 2 antibody
      • Eukaryotic translation elongation factor 2 antibody
      • Polypeptidyl tRNA translocase antibody
      • SCA26 antibody
      see all

    Anti-EEF2 antibody 图像

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma, using 1/100 ab84755 in conjunction with 1/100 Goat anti-Rabbit IgG conjugated with a fluorescent dye (red).
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human testicular seminoma, using 1/100 ab84755 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse teratoma, using 1/100 ab84755 followed by DAB staining.

    Anti-EEF2 antibody (ab84755)参考文献

    ab84755 has not yet been referenced specifically in any publications.

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