概述

  • 产品名称Anti-EEF2抗体
    参阅全部 EEF2 一抗
  • 描述
    兔多克隆抗体to EEF2
  • 经测试应用适用于: WB, ICC, Flow Cyt, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human EEF2.

  • 阳性对照
    • HEK293 and Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC 1/50 - 1/100.
Flow Cyt 1/20. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • 序列相似性Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • 翻译后修饰Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Anti-EEF2 antibody 图像

  • Anti-EEF2 antibody (ab40812) at 1/50000 dilution + 10ug HEK293

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ab40812 at a 1/100 dilution staining EEF2 in human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.

Anti-EEF2 antibody (ab40812)参考文献

This product has been referenced in:
  • Leal G  et al. Neuronal activity induces synaptic delivery of hnRNP A2/B1 by a BDNF-dependent mechanism in cultured hippocampal neurons. PLoS One 9:e108175 (2014). WB ; Rat . Read more (PubMed: 25286195) »
  • Sung VM & Tsai CL ADP-Ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates. BMC Biochem 15:26 (2014). WB . Read more (PubMed: 25494717) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse pancreatic acinar cells)
Loading amount 30 µg
Specification Mouse pancreatic acinar cells
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20 %)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Oct 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"