概述

  • 产品名称Anti-EEF1G抗体
    参阅全部 EEF1G 一抗
  • 描述
    兔多克隆抗体to EEF1G
  • 经测试应用适用于: ICC/IF, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide from a region between residue 200 and 250 of human EEF1G, NP_001395.1.

  • 阳性对照
    • Whole cell lysate from Hela, 293T and NIH3T3 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Probably plays a role in anchoring the complex to other cellular components.
  • 组织特异性Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
  • 序列相似性Contains 1 EF-1-gamma C-terminal domain.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2610301D06Rik antibody
    • AA407312 antibody
    • eEF 1B gamma antibody
    • EEF 1G antibody
    • eEF-1B gamma antibody
    • EEF1G antibody
    • EF 1 gamma antibody
    • EF 1G antibody
    • EF-1-gamma antibody
    • EF1 gamma antibody
    • EF1G antibody
    • EF1G_HUMAN antibody
    • Elongation factor 1 gamma antibody
    • Elongation factor 1-gamma antibody
    • Eukaryotic translation elongation factor 1 gamma antibody
    • GIG 35 antibody
    • GIG35 antibody
    • MGC103354 antibody
    • MGC114210 antibody
    • MGC144723 antibody
    • MGC144724 antibody
    • MGC94929 antibody
    • Pancreatic tumor related protein antibody
    • PRO1608 antibody
    • Translation elongation factor eEF 1 gamma chain antibody
    see all

Anti-EEF1G antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling EEF1G with ab72368 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EEF1G antibody (ab72368) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 30 seconds
  • Immunoprecipitation/ Western Blot of EEF1G.
    Lane 1: ab72368 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72368 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.
  • All lanes : Anti-EEF1G antibody (ab72368) at 1/3000 dilution (in PBS-T for 16 hours at 4°C)

    Lane 1 : Monkey kidney whole cell lysate overexpressing EEF1G
    Lane 2 : COS7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C

    See Abreview

  • IHC image of ab72368 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab72368 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72368, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-EEF1G antibody (ab72368)参考文献

This product has been referenced in:
  • Bellé R  et al. Identification of a new isoform of eEF2 whose phosphorylation is required for completion of cell division in sea urchin embryos. Dev Biol 350:476-83 (2011). WB ; Sea Urchin . Read more (PubMed: 21167828) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Monkey Cell lysate - whole cell (kidney)
Loading amount 20 µg
Specification kidney
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jul 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"