概述

  • 产品名称Anti-eEF1B2抗体
    参阅全部 eEF1B2 一抗
  • 描述
    小鼠单克隆抗体to eEF1B2
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Cow
  • 免疫原

    Recombinant fragment: VPSQADVAVF EAVSSPPPAD LCHALRWYNH IKSYEKEKAS LPGVKKALGK YGPADVEDTT GSG, corresponding to amino acids 29-91 of Human eEF1B2

  • 阳性对照
    • HeLa cell lysate. Recombinant tagged human eEF1B2 fragment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.

靶标

Anti-eEF1B2 antibody 图像

  • Anti-eEF1B2 antibody (ab77043) at 1 µg/ml + Recombinant tagged human eEF1B2 fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-eEF1B2 antibody (ab77043) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

Anti-eEF1B2 antibody (ab77043)参考文献

ab77043 has not yet been referenced specifically in any publications.

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