Rabbit, Chicken, Cow, Pig
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human eEF1B2.
(Peptide available as
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate, HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate, MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate and U2OS (Human osteosarcoma cell line) Whole Cell Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
Use a concentration of 1 µg/ml.
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
Belongs to the EF-1-beta/EF-1-delta family.
Contains 1 GST C-terminal domain.
Phosphorylation affects the GDP/GTP exchange rate.
Information by UniProt
Western blot - eEF1B2 antibody (ab37957)
All lanes :
Anti-eEF1B2 antibody (ab37957) at 1/250 dilution
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat whole cell lysate ( ab7899) Lane 3 : A431 whole cell lysate ( ab7909) Lane 4 : HEK293 whole cell lysate ( ab7902) Lane 5 : HepG2 whole cell lysate ( ab7900) Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution Performed under reducing conditions. Predicted band size: 25 kDa Observed band size: 30 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - eEF1B2 antibody (ab37957)
ICC/IF image of ab37957 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37957, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
has not yet been referenced specifically in any publications.
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