Anti-eEF1A1+eEF1A2+eEF1AL3抗体(ab37969)

概述

  • 产品名称Anti-eEF1A1+eEF1A2+eEF1AL3抗体
  • 描述
    兔多克隆抗体to eEF1A1+eEF1A2+eEF1AL3
  • 特异性Due to sequence homology the antibody recognises all three eEF1A forms, eEF1A1, eEF1A2 and eEF1A3. Through WB ab37969 was found to detect recombinant and endogenous eF1A1 and eF1A2.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Rabbit, Horse, Chicken, Hamster, Cow, Cat, Dog, Pig, Chimpanzee, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human eEF1A1.

    (Peptide available as ab37968.)

  • 阳性对照
    • This antibody gave a positive signal in the following: Human Whole Cell Lysates (HeLa, Jurkat, A431, HEK 293, HepG2, MCF-7, SHSY-5Y, U2OS) Mouse Whole Cell Lysates (NIH 3T3, MEF1) Mouse Tissue Lysates (Brain, Liver, Heart, Kidney, Pancreas, Testis, Skeletal muscle, Spinal Cord, Ovary) Rat Whole Cell Lysate(PC12) Rat Tissue Lysate (Brain, Liver, Heart)

性能

应用

Our Abpromise guarantee covers the use of ab37969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

靶标

  • 相关性Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Tissue specificity eEF1A1: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle. Tissue specificity eEF1A2: Brain, heart, and skeletal muscle. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. PTM eEF1A1: ISGylated.
  • 数据库链接
  • 别名
    • CCS3 antibody
    • eEF1A-1 antibody
    • eEF1A-2 antibody
    • EEF1A1 antibody
    • Eef1a2 antibody
    • EEF1AL antibody
    • EF 1 alpha 2 antibody
    • EF-1-alpha-1 antibody
    • EF-1-alpha-2 antibody
    • EF-Tu antibody
    • EF1A antibody
    • EF1A1_HUMAN antibody
    • EF1A2_HUMAN antibody
    • Elongation factor 1 A 2 antibody
    • Elongation factor 1 alpha antibody
    • Elongation factor 1 alpha 2 antibody
    • Elongation factor 1-alpha 1 antibody
    • Elongation factor 1-alpha 2 antibody
    • Elongation factor Tu antibody
    • Eukaryotic elongation factor 1 A-1 antibody
    • Eukaryotic elongation factor 1 A-2 antibody
    • Eukaryotic translation elongation factor 1 alpha 2 antibody
    • GRAF 1EF antibody
    • HS1 antibody
    • Leukocyte receptor cluster member 7 antibody
    • PTI1 antibody
    • Statin-S1 antibody
    see all

Anti-eEF1A1+eEF1A2+eEF1AL3 antibody 图像

  • All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)
    Lane 5 : HepG2 whole cell lysate (ab7900)
    Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
  • ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 5 : Heart (Mouse) Tissue Lysate
    Lane 6 : Kidney (Mouse) Tissue Lysate
    Lane 7 : Mouse pancreas tissue lysate - total protein (ab29363)
    Lane 8 : Testis (Mouse) Tissue Lysate
    Lane 9 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 10 : Spinal Cord (Mouse) Tissue Lysate
    Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 14 : Liver (Rat) Tissue Lysate
    Lane 15 : Heart (Rat) Tissue Lysate
    Lane 16 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml

    Lane 1 : Recombinant Human eEF1A1 protein (ab81792)
    Lane 2 : Human EEF1A2 full length protein (ab40317)

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 3 minutes

Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)参考文献

This product has been referenced in:
  • Dong C  et al. Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity. J Neurosci 34:3171-82 (2014). Read more (PubMed: 24573276) »
  • Hashimoto K & Ishima T Neurite Outgrowth Mediated by Translation Elongation Factor eEF1A1: A Target for Antiplatelet Agent Cilostazol. PLoS One 6:e17431 (2011). WB ; Rat . Read more (PubMed: 21390260) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spinal Cord)
Permeabilization No
Specification Spinal Cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Herr Dr. Ralph Nawrotzki

Verified customer

提交于 Aug 17 2015

Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

Credit note ID: 23754

The credit note...

Read More

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ...

Read More

Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Although the other antibody ...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

Read More

Thank you for your reply, and I apologize again for this error. I found your order *** from October 2008, and I have asked our finance department to issue credit note #***. The credit note may be used in one of the following ways: (1) R...

Read More

Thank you for your reply. The PO# on this order is 1244827. I appreciate you forwarding the email to the end user. Please let me know if you need any more information from me.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A549 lung cells)
Specification A549 lung cells
Fixative Formaldehyde
Permeabilization Yes - 1% TX-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. David MAtthews

Verified customer

提交于 Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"