重组Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3抗体[EPR9471] (ab157455)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9471] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3抗体[EPR9471]
参阅全部 eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 一抗 -
描述
兔单克隆抗体[EPR9471] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 6 continuous identical amino acids with eEF1A2. Cross-reactivity with this protein has not been confirmed experimentally. -
经测试应用
适用于: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, MCF7, 293T and Neuro-2a cell lysates. GST tagged Recombinant Human EEF1A1 and EEF1AL3 protein
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9471 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab157455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/40000. Predicted molecular weight: 50 kDa.
For unpurified use at 1/1000 - 1/10000. |
IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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WB
1/40000. Predicted molecular weight: 50 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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细胞定位
eEF1A1/EF-Tu: Cytoplasm. -
数据库链接
- Entrez Gene: 1915 Human
- Entrez Gene: 13627 Mouse
- Entrez Gene: 171361 Rat
- Omim: 130590 Human
- SwissProt: P68104 Human
- SwissProt: P10126 Mouse
- SwissProt: P62630 Rat
- Unigene: 520703 Human
see all
图片
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All lanes : Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab157455) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human EEF1A1 protein (Full length, 76 KDa)
Lane 2 : His tagged Recombinant Human EEF1AL3 protein (Full length, 52 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 50 kDaExposure time:
Lane 1: 180 seconds
Lane 2: 5 seconds
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All lanes : Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab157455) at 1/40000 dilution (purified)
Lane 1 : Rat kidney lysate
Lane 2 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 50 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling eEF1A1/EF-Tu with purified ab157455 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling eEF1A1/EF-Tu with purified ab157455 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling eEF1A1/EF-Tu with purified ab157455 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab157455) at 1/40000 dilution (purified) + Mouse kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 50 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST. -
All lanes : Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab157455) at 1/50000 dilution (purified)
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20 µg (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 50 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9471] (ab157455) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa -
ab157455 (purified) at 1/30 immunoprecipitating eEF1A1/EF-Tu in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab157455 被引用在 8 文献中.
- Sobočan M et al. Initiation and elongation factor co-expression correlates with recurrence and survival in epithelial ovarian cancer. J Ovarian Res 15:73 (2022). PubMed: 35718769
- Duman M et al. EEF1A1 deacetylation enables transcriptional activation of remyelination. Nat Commun 11:3420 (2020). PubMed: 32647127
- Qin Y et al. Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells. Mol Ther Nucleic Acids 21:492-511 (2020). PubMed: 32679544
- Zhou H et al. Evaluation on Elongation Factor 1 Alpha of Entamoeba histolytica Interaction with the Intermediate Subunit of the Gal/GalNAc Lectin and Actin in Phagocytosis. Pathogens 9:N/A (2020). PubMed: 32867020
- Mazaré N et al. Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning. Cell Rep 32:108076 (2020). PubMed: 32846133
- Ma SY et al. Disruption of Plin5 degradation by CMA causes lipid homeostasis imbalance in NAFLD. Liver Int 40:2427-2438 (2020). PubMed: 32339374
- Liu F et al. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell 167:816-828.e16 (2016). WB ; Human . PubMed: 27745969
- Li S et al. eEF1A Interacts with the NS5A Protein and Inhibits the Growth of Classical Swine Fever Virus. Viruses 7:4563-81 (2015). PubMed: 26266418