概述

  • 产品名称Anti-EED抗体
    参阅全部 EED 一抗
  • 描述
    兔多克隆抗体to EED
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis , Medaka fish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 21-70 (QKLSSDENSN PDLSGDENDD VSIESGTNTE PDTPTNTPNA GRKSWGK) of Human EED (NP_694536)

  • 阳性对照
    • HepG2 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab113911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • 组织特异性Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
  • 序列相似性Belongs to the WD repeat ESC family.
    Contains 7 WD repeats.
  • 发展阶段Expression peaks at the G1/S phase boundary.
  • 细胞定位Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • eed antibody
    • EED protein antibody
    • EED, mouse, homolog of antibody
    • EED_HUMAN antibody
    • Embryonic ectoderm development antibody
    • Embryonic ectoderm development isoform a antibody
    • Embryonic ECTODERM development protein homolog antibody
    • Embryonic ectoderm development protein, mouse, homolog of antibody
    • hEED antibody
    • OTTHUMP00000235483 antibody
    • OTTHUMP00000235484 antibody
    • OTTHUMP00000235485 antibody
    • Polycomb protein eed antibody
    • WAIT 1 antibody
    • WAIT-1 antibody
    • WAIT1 antibody
    • WD protein associating with integrin cytoplasmic tails 1 antibody
    see all

Anti-EED antibody 图像

  • Anti-EED antibody (ab113911) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 45 kDa

Anti-EED antibody (ab113911)参考文献

ab113911 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Chicken Tissue lysate - whole (brain)
Loading amount 5.2 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Ying Jiang

Verified customer

提交于 Oct 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"