RabMAb

Anti-EEA1抗体[EPR4245] - Early Endosome Marker (ab109110)

概述

性能

应用

Our Abpromise guarantee covers the use of ab109110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 162 kDa).
ICC 1/500 - 1/1000.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • 序列相似性Contains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • 结构域The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • 细胞定位Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Anti-EEA1 antibody [EPR4245] - Early Endosome Marker 图像

    • All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilution

      Lane 1 : COS-1 cell lysate
      Lane 2 : NIH 3T3 cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 162 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)

    Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)参考文献

    ab109110 has not yet been referenced specifically in any publications.

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