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RabMAb

Anti-EEA1抗体[EPR4245] - Early Endosome Marker (ab109110)

概述

  • 产品名称
    Anti-EEA1抗体[EPR4245] - Early Endosome Marker
    参阅全部 EEA1 一抗
  • 描述
    兔单克隆抗体[EPR4245] to EEA1 - Early Endosome Marker
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ICCmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the N terminus of Human EEA1 (UniProt Q15075).

  • 阳性对照
    • COS-1, NIH 3T3, C6, HeLa, Jurkat, and JAR cell lysates
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 162 kDa).
ICC 1/500 - 1/1000.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • 序列相似性
      Contains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • 结构域
      The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • 细胞定位
      Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    图片

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Early Endosome Marker knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: NIH3T3 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109110 observed at 162 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab109110 was shown to recognize Early Endosome Marker in wild-type HAP1 cells as signal was lost at the expected MW in Early Endosome Marker knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Early Endosome Marker knockout samples were subjected to SDS-PAGE. Ab109110 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilution

      Lane 1 : COS-1 cell lysate
      Lane 2 : NIH 3T3 cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 162 kDa
      Observed band size: 170 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Wolff NA  et al. Evidence for mitochondrial localization of divalent metal transporter 1 (DMT1). FASEB J 28:2134-45 (2014). Read more (PubMed: 24448823) »
    • Choi S  et al. Globotriaosylceramide induces lysosomal degradation of endothelial KCa3.1 in fabry disease. Arterioscler Thromb Vasc Biol 34:81-9 (2014). Read more (PubMed: 24158513) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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