兔单克隆抗体[EPR4245] to EEA1 - Early Endosome Marker
Mouse, Rat, Human
Synthetic peptide corresponding to residues in the N terminus of Human EEA1 (UniProt Q15075).
COS-1, NIH 3T3, C6, HeLa, Jurkat, and JAR cell lysates
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This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 162 kDa).
1/500 - 1/1000.
Is unsuitable for Flow Cyt,IHC-P or IP.
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Contains 1 C2H2-type zinc finger.
Contains 1 FYVE-type zinc finger.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
Cytoplasm. Early endosome membrane.
Information by UniProt
Early endosome antigen 1 antibody
Early endosome antigen 1, 162kD antibody
Early endosome associated protein antibody
Western blot - EEA1 antibody [EPR4245] (ab109110)
All lanes :
Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilution
Lane 1 :
COS-1 cell lysate
Lane 2 :
NIH 3T3 cell lysate
Lane 3 :
C6 cell lysate
Lane 4 :
HeLa cell lysate
Lane 5 :
Jurkat cell lysate
Lane 6 :
JAR cell lysate
Lysates/proteins at 10 µg per lane.
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size :
Observed band size :
170 kDa (
why is the actual band size different from the predicted?
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