概述

  • 产品名称Anti-EEA1抗体
    参阅全部 EEA1 一抗
  • 描述
    兔多克隆抗体to EEA1
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 1361-1411 (CGKGFSVTVR RHHCRQCGNI FCAECSAKNA LTPSSKKPVR VCDACFNDLQ G) of Human EEA1, NP_003557.2

  • 阳性对照
    • Whole cell lysate from HeLa cells, 293T cells and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab86566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • 序列相似性Contains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • 结构域The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • 细胞定位Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Anti-EEA1 antibody 图像

    • All lanes : Anti-EEA1 antibody (ab86566) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


      Predicted band size : 162 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    Anti-EEA1 antibody (ab86566)参考文献

    ab86566 has not yet been referenced specifically in any publications.

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