概述

  • 产品名称
  • 描述
    兔多克隆抗体to EEA1
  • 经测试应用
    适用于: WB, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    GSQGSDLDSSATPINTVDVNNESSSEG

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1

  • 阳性对照
    • whole extract of mouse NIH-3T3 cells; human HeLa and rat NRK cells.

性能

应用

Our Abpromise guarantee covers the use of ab50313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 5 - 10 µg/ml.
    WB: Use at a concentration of 0.4 - 0.8 µg/ml. Detects a band of approximately 160 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • 序列相似性
      Contains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • 结构域
      The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • 细胞定位
      Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    图片

    • HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."
    • ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.

      See Abreview

    文献

    This product has been referenced in:
    • Acharya M  et al. av Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells. Nat Commun 7:10917 (2016). Read more (PubMed: 26965188) »
    • Xia X  et al. Porous silicon microparticle potentiates anti-tumor immunity by enhancing cross-presentation and inducing type I interferon response. Cell Rep 11:957-966 (2015). Read more (PubMed: 25937283) »

    See all 11 Publications for this product

    客户评价及客户问答

    Thank you very much for your responses.
    I'm sending a free of charge vial of ab95195 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this antibody.
    For the replacement of ab50313, I certainly und...

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    Thank you very much for your reply and for sending all of this information.
    I will be happy to send the replacement ab95195, but I will need the original order number or PO number that ab2074 was received on. Would you be able to provide this info...

    Read More

    Thanks for the update. We certainly appreciate your lab's troubleshooting efforts. Please feel free to reply after your trip.

    We have previously been in contact regarding ab2074, which was not providing satisfactory results. We had offered a free of charge replacement with a different antibody, but we were waiting to hear from you with your selection. If you have dete...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (primary astrocyte)
    Specification
    primary astrocyte
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% saponin
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 08 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Lung carcinoma cell line)
    Specification
    Lung carcinoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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