Anti-EDG6/S1PR4抗体(ab92993)
Key features and details
- Rabbit polyclonal to EDG6/S1PR4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EDG6/S1PR4抗体
参阅全部 EDG6/S1PR4 一抗 -
描述
兔多克隆抗体to EDG6/S1PR4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog, Pig, Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human EDG6/S1PR4 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab126638) -
阳性对照
- This antibody gave a positive signal in Jurkat whole cell lysate as well as the following tissue lysates: Human Spleen; Human Tonsil; Human Lymph node.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92993于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa). |
靶标
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功能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes. -
组织特异性
Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 539534 Cow
- Entrez Gene: 101150342 Gorilla
- Entrez Gene: 8698 Human
- Entrez Gene: 100517997 Pig
- Omim: 603751 Human
- SwissProt: O95977 Human
- Unigene: 662006 Human
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别名
- EDG6 antibody
- Endothelial differentiation lysophosphatidic acid G protein coupled receptor 6 antibody
- Endothelial differentiation G protein coupled receptor 6 antibody
see all
图片
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All lanes : Anti-EDG6/S1PR4 antibody (ab92993) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Tonsil (Human) Tissue Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesEDG6/S1PR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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