概述

  • 产品名称Anti-EDG4抗体
    参阅全部 EDG4 一抗
  • 描述
    兔多克隆抗体to EDG4
  • 特异性This antibody is specific for EDG4 (N term).
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    NETIGFFYNNSGKELSSHWRP

    conjugated to KLH, corresponding to N terminal amino acids 10/30 of Human EDG4

  • 阳性对照
    • Breast carcinoma tissue and HL60 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab38322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/25 - 1/100.
WB 1/100 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

靶标

  • 功能Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation.
  • 组织特异性Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA colocalizes with RALA in the endocytic vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EDG 4 antibody
    • EDG-4 antibody
    • EDG4 antibody
    • Endothelial Cell Differentiation Gene 4 antibody
    • Endothelial differentiation lysophosphatidic acid G protein coupled receptor 4 antibody
    • FLJ93869 antibody
    • G protein coupled receptor antibody
    • IPA2 antibody
    • LPA 2 antibody
    • LPA receptor 2 antibody
    • LPA receptor EDG4 antibody
    • LPA-2 antibody
    • LPA2 antibody
    • LPAR2 antibody
    • LPAR2_HUMAN antibody
    • Lysophosphatidic acid receptor 2 antibody
    • Lysophosphatidic acid receptor Edg 4 antibody
    • Lysophosphatidic acid receptor Edg-4 antibody
    • Lysophosphatidic acid receptor EDG4 antibody
    see all

Anti-EDG4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling EDG4 with ab38322 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-EDG4 antibody (ab38322) at 1/1000 dilution + HL-60 whole cell lysate at 35 µg/ml

    Secondary
    goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 15 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-EDG4 antibody (ab38322)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample Mouse Tissue sections (Cortical)
Specification Cortical
Permeabilization Yes - 0.3% TritonX
Fixative Paraformaldehyde
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提交于 Jun 24 2013

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I am sorry that this antibody did not perform as stated on the datasheet. If payment ha...

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Thank you very much for your interest in ab38322.

To our knowledge, ab38322 has not been tested in Flow cytometry and we do not offer samples. Therefore, I can offer a discount off a future purchase if you buy ab38322 now, test it in Flow cy...

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Merci de nous avoir contactés. Je peux confirmer que pour tester cet anticorps sur du tissue en paraffine, un démasquage au tampon Citrate pH 6 avait été utilisé. Veuillez trouver le protocole en attaché. Je peux aussi conseiller de con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"