EDG2 / LPA-1 overexpression 293T裂解物(whole cell) (ab94195)
概述
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产品名称
EDG2 / LPA-1 overexpression 293T裂解物(whole cell)
参阅全部 EDG2 /LPA-1 细胞裂解液 -
常规说明
ab94195 is a 293T cell transfected lysate in which Human EDG2 / LPA-1 has been transiently over-expressed using a pCMV-EDG2 / LPA-1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. Tissue specificity: Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes. Similarity: Belongs to the G-protein coupled receptor 1 family.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94195于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94195 at 15μg/lane on an SDS-PAGE gel
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All lanes : Anti-EDG2 /LPA-1 antibody (ab89628) at 1/500 dilution
Lane 1 :EDG2 / LPA-1 overexpression 293T lysate (whole cell) (ab94195)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94195 尚未被引用在任何文献中。