Anti-EDG1 (phospho T236)抗体(ab111571)

概述

  • 产品名称Anti-EDG1 (phospho T236)抗体
    参阅全部 EDG1 一抗
  • 描述
    兔多克隆抗体to EDG1 (phospho T236)
  • 特异性ab111571 detects endogenous levels of EDG1 only when phosphorylated at threonine 236 (Human: Thr236; Mouse: Thr236; Rat: Thr237).
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human EDG1 around the phosphorylation site of Threonine 236 (R-L-TP-F-R).

  • 阳性对照
    • COS-7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab111571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/500.

靶标

  • 功能Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
  • 组织特异性Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD363 antibody
    • CHEDG 1 antibody
    • CHEDG1 antibody
    • D1S3362 antibody
    • ECGF 1 antibody
    • ECGF1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • endothelial differentiation G protein coupled receptor 1 antibody
    • Endothelial differentiation G-protein coupled receptor 1 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 1 antibody
    • FLJ58121 antibody
    • G protein coupled sphingolipid receptor antibody
    • g protein-coupled receptor edg-1 antibody
    • S1P receptor 1 antibody
    • S1P receptor Edg 1 antibody
    • S1P receptor Edg-1 antibody
    • S1P receptor Edg1 antibody
    • S1P(1) receptor antibody
    • S1P1 antibody
    • s1pr1 antibody
    • S1PR1_HUMAN antibody
    • sphingolipid g-protein-coupled receptor 1 antibody
    • Sphingosine 1 phosphate receptor Edg 1 antibody
    • Sphingosine 1 phosphate receptor EDG1 antibody
    • sphingosine 1- phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor Edg-1 antibody
    • Sphingosine 1phosphate receptor type 1 S1P1 antibody
    see all

Anti-EDG1 (phospho T236) antibody 图像

  • ab111571, at 1/100 dilution, staining EDG1 in COS-7 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-EDG1 (phospho T236) antibody (ab111571)参考文献

ab111571 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Human Cell (HEK293 overexpressing GFP tagged EDG1)
Specification HEK293 overexpressing GFP tagged EDG1
Permeabilization Yes - 0.1% triton X100 in PBS
Fixative Paraformaldehyde
Username

Mr. Martin Baker

Verified customer

提交于 Nov 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"